Screening Differential Expression Profiles of Urinary microRNAs in a Gentamycin-Induced Acute Kidney Injury Canine Model
نویسندگان
چکیده
microRNAs (miRNAs) are promising biomarkers for different pathological models because of their stable and detectable characters in biofluids. Here, we collected urine samples from 5 beagle dogs on the 3th, 6th, 12th day an acute kidney injury (AKI) caused by gentamycin. miRNA levels were measured with high-throughput sequencing results then differentially investigated. Gene Ontology (GO) KEGG pathway analysis performed to analyze potential target genes corresponding expressed miRNAs (DE-miRNAs). Relationships between hub DE-miRNAs analyzed STRING Cytoscape. We identified 234 3, 6, 12 days after gentamycin treatment (p < 0.05). Top 10 up- down-regulated candidate predicted overlapping TargetScan miRanda results). GO analyses demonstrated that mainly involved injury-related pathways, such as insulin signaling pathway, oxytocin hedgehog pathway. The network miRNA-hub suggests miR-452, miR-106a, 106b participate regulating largest number genes. evaluated signature via a canine model built gentamycin-caused injury. Our represent valuable resource evaluating renal toxicity.
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ژورنال
عنوان ژورنال: Kidney and dialysis
سال: 2023
ISSN: ['2673-8236']
DOI: https://doi.org/10.3390/kidneydial3020019